Figure 1

RT-PCR for CD4, CCR3, CCR5, CCR8, CXCR4 and β actin mRNA detection in KS cells. RT-PCR was performed on total RNA extracted from KS-2 cells at early and late passages (passages 4 and 12, respectively) and from KS-Y1 cells, as described in the Materiel and Methods section. Lanes 1, non-stimulated cells; lanes 2 and 3, cells were stimulated for 8 hours with 80 pg/ml IL-1β (lanes 2) or 4 hours with 1 μg/ml LPS (lanes 3); lanes 4, positive controls for RT-PCR, consisting in RT-PCR performed on total RNA extracted from T cells (positive controls for CD4, CCR8, CXCR4 and β actin), LPS-stimulated macrophages (positive control for CCR5) and CHO cells transfected with the CCR3 gene (positive control for CCR3). Similar results were obtained with KS-1, -3, -4 and -5 cells (data not shown).