Figure 3

COCA keratinocytes regenerate an epidermis-like structure in vivo upon grafting. COCA keratinocytes in passage 77 were grafted into silicon chambers placed in the back skin of immunodeficient mice. H&E (A) and immunofluorescence (B-F) staining of the epidermis formed 4 weeks after grafting. B&C) K5 was expressed mainly by basal keratinocytes but displayed some expansion to suprabasal layers; however, BrdU-K5 positive cells were located mainly in the basal layer (B). D) K6 was present in basal and suprabasal layers and overlapped partially with the expression of the early differentiation marker K10. C-F) K10 was not expressed in the basal layer. Loricrin (E) and filaggrin (F) were expressed in suprabasal layers. Scale bars: 100 μm.