Figure 2

Growth and expansion of ePUK culture. 2a: Ten T-75 flasks were produced in 10 days, one per day from one P1 flask. The P1Sn flasks became confluent, producing P1Snpn flasks, continuing to form additional generations of ePUKs for several months. 2b: This Figure presents the plating efficiency of the passages from Figure 2a. Passages P1S1 to P1S5, showed good plating efficiency reaching confluence after 3–4 days, while passages P1S6 to P1S10 demonstrate the decline of the original P1 flask and the vigor and viability of the ePUKs at the end of the 2 week period. This decline was always seen, as each generation of the cell line reach confluence, produced ePUKs and then became an “exhausted” monolayer. The ePUKs were established from urothelium.