Figure 5
Immunohistochemical staining of ePUK cells for epithelial cell markers. Anti-Ki67, -p63, involucrin, and -cd71 antibodies are testing epidermal derived ePUKs; anti-k14, -k10, and -α6β-4 integrin antibodies are testing keratinized mucosa derived ePuks and anti-loricrin antibody is testing non-keratinized mucosal ePUKs. Positive staining is brown, and counter stain is blue. The ePUK cells are identified as cycling (+ for Ki67) basal cells (+ for p63 and K14). Non-keratinized oral mucosal is negative for Loricrin, and epidermal ePUKs showed staining for involucrin. Bar = 50 micron.