Skip to main content
Figure 4 | BMC Dermatology

Figure 4

From: Functional expression of NF1 tumor suppressor protein: association with keratin intermediate filaments during the early development of human epidermis

Figure 4

Avidin-biotin immunolabeling of NF1 protein and in situ hybridization of NF1 mRNA in developing human epidermis. (a) At 13 weeks EGA, the basal and the periderm cell layers are intensely labeled with the NF1GRP(D) antibody. Also intermediate cells show positive reaction near cell membranes. (b) Hematoxylin-eosin staining of developing epidermis at 13 weeks EGA. (c) NF1 mRNA is noted in the basal and intermediate cell layers at 13 weeks EGA. Subcellularly, NF1 mRNA is located at the cell periphery of intermediate cells (arrowhead). Note the positive NF1 mRNA signal at the basal aspect of basal cells at this stage of development. Arrows point to the basement membrane zone. (d) At 18 weeks EGA, the basal cells display the most intense immunoreaction with NF1GRP(D) antibody. (e) Hematoxylin-eosin staining of 18-week-old epidermis. (f) At 18 weeks EGA, NF1 mRNA is detectable only in the suprabasal cell layer by in situ hybridization. The positive NF1 mRNA signal is located near cell membranes (arrowhead). Arrows point to the basement membrane zone. Scale bars 20 μm.

Back to article page

BMC Dermatology

ISSN: 1471-5945

Contact us