Figure 2

Expression of Notch1 , Jagged1 and Lunatic Fringe genes in basal cell carcinoma and in experimental wound healing. Propidium iodide nuclei counterstaining (A-C, D-F) and in situ hybridisation with radiolabelled riboprobes (A'-C', D'-F'). In superficial basal cell carcinoma (A-C') Notch1, Jagged1 and Lunatic Fringe transcripts present a low level of expression in un-proliferating epidermis (filled arrowheads), and no expression in palisading cells embedded in the dermis (empty arrowheads). In wound healing experiments (D-F') gene expression in regenerated epidermis is lower in the middle (empty arrowheads) than at the wound edges (filled arrowheads). Scale bar, 100 μm; d: dermis; e: epidermis; g: granulation tissue; he: hyperproliferative epidermis; s: scab; Lu, Lunatic.