Figure 2From: Enhanced diagnostic immunofluorescence using biopsies transported in salineDirect immunofluorescence (IgG, combined with transmitted light) in saline transported skin specimen of lupus erythematosus. After 48 hours in saline there is subepidermal split formation, not present in fresh-frozen (N2) and fixed (Mi48) skin. Note the still obvious granular IgG fluorescence at the dermal side of the split. (obj. ×40)Back to article page