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Table 1 Results of direct immunofluorescence (DIF) of matched skin biopsies transported in different media.

From: Enhanced diagnostic immunofluorescence using biopsies transported in saline

  

N2

Mi48

S24

S48

Diagnosis by DIF

DSS

n = 25

n = 22§

n = 25

n = 23§

a. conclusive

+/+++

14 (56%)

11 (50%)

21 (84%)

16 (70%)

b. tentative

±/+

5 (20%)

4 (18%)

2 (8%)

3 (13%)

c. non-diagnostic

-/±

5 (20%)

7 (32%)

2 (8%)

4 (17%)

d. mis-diagnosed

 

1 (4%)

-

-

-

  1. N2, snap-frozen specimens in liquid nitrogen; Mi48, specimens in Michel's fixative for 48 hours; S24 and S48, specimens in saline for 24 and 48 hours respectively; DSS, desired specific staining; n, number of cases
  2. § Two S48 and three Mi48 matched biopsies were either lacking or considered unsuitable.