Table 1 Overview about the performed immunohistochemistry (alkaline phosphatase anti-alkaline phosphatase (APAAP) technique using the labelled streptavidin-biotin (LSAB) method)
Antibody | Source | Pretreatment* | Dilution | Incubation time |
|---|---|---|---|---|
CD4 | Novocastra Loxo, Dossenheim, Germany | H | 1:60 | 30 min |
CD68 | Dako, Hamburg, Germany | P | 1:25 | 30 min |
Tryptase | Dako, Hamburg, Germany | P | 1:400 | 28 min |
MIB-1 | Dako, Hamburg, Germany | H | 1:10 | 32 min |
FGF-R | Oncogene Research, San Diego, USA | N | 1:10 | 30 min |
Decorin | Oncogene Research, San Diego, USA | N | 1:10 | 30 min |
CD20 | Novocastra Loxo, Dossenheim, Germany | H | 1:50 | 30 min |