Figure 4

Transcriptional activity of TGM1 distal promoter (A) At the top is shown a map of the TG 2.2 kb promoter with AP1 and CRE sites indicated by arrowheads. Numbers are distances in kilobase pairs from the transcription start site (0). Shown below are schematics of 5' and internal deletions. Internal deletion constructs are named according to the regions of the distal promoter included. All promoters contained a small segment of the proximal promoter and the transcription start site (-90 to +67) and were fused to the firefly luciferase gene in plasmid pGL3. (B) Transcriptional activities of the depicted promoters were determined by transient transfection into rB (white bars) and SIK (filled bars) cell lines. Firefly luciferase activity was divided by the activity of a cotransfected CMV Renilla plasmid to correct for different transfection efficiencies and the result was expressed as the percentage of activity of the TG 2.2 plasmid. The means ± SD of 2–10 independent experiments, each done in duplicate, are shown.