Construct | Cell Line Transfected |
|---|
| Â | hEp | SIK | rEp | rB |
TG 1.6 | 112 ± 26 | 86 ± 24 | 85 ± 19 | 85 ± 18 |
TG 1.5 | 46 ± 6 | 27 ± 8 | 59 ± 7 | 56 ± 17 |
TG 0.54 | 4 ± 4 | 7 ± 6 | 12 ± 1 | 9 ± 5 |
TG 2.2-0.5 | 54 ± 1 | 81 ± 12 | 49 ± 17 | 82 ± 5 |
TG 2.2-1.0 | 51 ± 6 | 55 ± 13 | Not done | 69 ± 37 |
TG 2.2-1.5 | * | 15 ± 11 | 7 ± 9 | 6 ± 4 |
- The constructs used for transfection are depicted and described in Fig 4, except that the 3' deletions used in this table extended to -2.2 kb instead of -1.6 kb. Firefly luciferase activity was divided by the activity of a cotransfected CMV Renilla plasmid to correct for different transfection efficiencies. To assess only the contribution of upstream sequences, the low amount of activity contributed by the proximal promoter (0.09 kb) was subtracted, and the result was expressed as the percentage of activity of the TG 2.2 plasmid. The means ± SD of 2–7 independent experiments, each done in duplicate, are shown. * Values were lower than minimal promoter alone.
