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Figure 2 | BMC Dermatology

Figure 2

From: Calcium and Vitamin D increase mRNA levels for the growth control hIK1 channel in human epidermal keratinocytes but functional channels are not observed

Figure 2

Consistent hIK1 mRNA levels among biopsies from individuals and donor groups are maintained in explant culture. Real-time PCR analysis of mRNA expression levels (normalized to GAPDH) in human skin biopsies from healthy donors. (a) data points are hIK1 mRNA levels from three biopsies from each of three donors with mRNA harvesting performed at time of biopsy collection. There were no statistically significance differences in hIK1 expression among the three donors. (b) effects of culture time on gene expression in explant biopsies assessed by measuring mRNA levels from biopsies preserved in RNALater at time of collection, and assessing fold changes from these levels in sibling biopsies shipped overnight in culture medium (1 day post collect), or shipped and kept in culture for 1 and 2 additional days (2 and 4 days post collect). Expression of involucrin (INV) and transglutaminase (TG-1) are shown for comparison with hIK1. Each data point is mean ± SEM for 3 biopsies. For any given gene there were no statistically significant differences in expression when comparing data from days one, two, and four.

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BMC Dermatology

ISSN: 1471-5945

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