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Figure 5 | BMC Dermatology

Figure 5

From: Calcium and Vitamin D increase mRNA levels for the growth control hIK1 channel in human epidermal keratinocytes but functional channels are not observed

Figure 5

Both NIH3T3 cells and calcium/VitD-treated keratinocytes have abundant IK1 mRNA but only NIH3T3 display IK1 currents. Top panels show whole cell patch clamp recordings from a keratinocyte (treated 5 days with 2 mM calcium and 1 μM VitD) and ras-transformed NIH3T3 fibroblast. Each panel comprises records taken prior to and then during 1–2 minute application of 10 μM A23187, a calcium ionophore which allows IK channel activity to be observed during voltage steps to 0 mV (voltage protocol shown beneath current records). mIK1 current is readily observed in ras-transformed NIH3T3 cells in response to A23187, while hIK1 activity is absent from keratinocytes. Bottom panel shows real-time PCR analysis of IK mRNA levels from sibling cultures to those used for the patch clamp recordings (mIK1 for NIH3T3, hIK1 for keratinocytes). Each column represents mean ± SEM of two cultures. For comparison filled columns show mRNA levels for wild type (wt, non-transformed) NIH3T3 cells and untreated keratinocytes (0.06 mM calcium, no VitD).

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