Figure 6

End-point PCR for the full-length protein coding sequence of hIK1 confirms real-time PCR results. Conventional end-point RT-PCR amplifying the coding sequence (1281 bp of 1284) of hIK1 was performed on keratinocytes grown for five days in either proliferative (0.06 mM calcium) or anti-proliferative, pro-differentiating (2 mM calcium, 1 μM VitD) culture conditions. GAPDH, 615 bp of the complete coding sequence, is shown as a loading control. Full-length hIK1 message is upregulated by VitD and more so by combining VitD with calcium, exactly as for the real-time PCR results (see figure 1).