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Table 1 Mouse ear swelling tests (MESTs): positive control + three cyanobacteria suspensions + purified cylindrospermopsin

From: Primary irritant and delayed-contact hypersensitivity reactions to the freshwater cyanobacterium Cylindrospermopsis raciborskii and its associated toxin cylindrospermopsin

TEST MATERIAL

Proportion of test mice with ear swelling >20% after challenge

Proportion of test mice with ear swelling >20% after re-challenge

Per cent ear swelling of test group after challenge. Median (range)

Per cent ear swelling of test group after re-challenge. Median (range)

Per cent ear swelling of highest responder control mouse after challenge and re-challenge

Fisher's exact test comparing proportion of ear thickness increases <20% between test and control mice

0.1% DNCB

100% n= 10 at 24 hrs

n = 9 at 24 & 48 hrs

Not done

68.6 (16.4 – 130.0)

Not done

Not done

 

2% w/v M. aeruginosa microcystins 13.6 μg/mL

0

0

1.9 (-11.6 – 10.7)

1.2 (-1.6 – 10.0)

8.9

 

2% w/v A. circinalis saxitoxins 6.0 μg/mL

0

0

0.0 (-6.8 – 8.2)

1.5 (-2.9 – 9.5)

11.7

 

2% w/v C. raciborskii cylindrospermopsin 73 μg/mL

60%

n= 2 at 24 hrs

n= 4 at 48 hrs

60%

n = 6 at 24 hrs

n = 2 at 24 & 48 hrs

15.8 (0.0 – 38.9)

18.3 (2.2 – 47.5)

10.7

p = 0.001

100 μg/mL cylindrospermopsin*

22%

n = 1 at 24 hrs

n = 1 at 48 hrs

44%

n = 1 at 24 hrs

n = 3 at 48 hrs

8.1 (-4.8 – 26.1)

12.6 (4.5 – 49.2)

4.8 (challenge)

27.8 (re-challenge)

p = 0.51 (challenge)

p = 0.58 (rechallenge)

  1. * challenge concentration applied to test ears was 50 μg/mL. Rechallenge concentration was 100 μg/mL (= induction concentration).