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Table 1 Primer sequences, PCR conditions and restriction enzymes used for mutation analysis and genotyping of selected polymorphisms in the TOLLIP gene.

From: Association of toll-interacting protein gene polymorphisms with atopic dermatitis

Amplicon

Rs number

Amino acid

Primer

Annealing temperature

MgCl2 (mmol/l)

Restriction enzyme

TOLLIP-526 C/G

5743854

-

F: GGGTGACCTTCGGACGTG

R: CATCTTTAGCCCTGTGAAACAG

57°C

2

Hin6I

TOLLIP exon 1*

-

-

F: GACAACTGTCTCCGTCGCA

R: TGGGTCAGGGCTACTGCT

55°C

2

-

TOLLIP intron1a

5743867

-

F: ACTCTCCCAGCACCTGACGCTTAAG

R: ACCAGCGCTACCAACTGTGGCGAT

61°C

4

MboI

TOLLIP intron1b

11042484

-

F: GACCCAGCCGTCTGCAGGG

R: TCCATGGCACAGGCAGGTACG

62°C

2

Hin6I

TOLLIP exon 2*

-

-

F: ACCCTGACTCACTGGCCTC

R: TTTCAACATCACACAGGTTTCA

58°C

2

-

TOLLIP exon 3*

-

-

F: GTTGGCACCTCCTTTTCCTGAC

R: CAGGTGTGTCCCCACGGC

66°C

2

-

TOLLIP exon 4

3750920

Pro139Pro

F: GGGCCTCTCCCTCTTGTC

R: TCAGAACGTGAGCCCTGC

60°C

3

MspI

TOLLIP exon 5*

-

-

F: GGTTCTGATGGGTGGATGTTCC

R: ACCTGCCCTAGGCCCTGG

66°C

2

-

TOLLIP exon 6

5744015

Ala222Ser

F: TTCCGACGTTTTTACCAAACTTGAT

R: GCGTGTCCAAAGAGCGGG

63°C

2

MwoI

TOLLIP 3'UTR

3750919

-

F: AGCCTGACTCTCACACTCAGCA

R: GGGAGCAAAACCTTGATACCAA

58°C

3

Alw26I

  1. F = forward primer, R = reverse primer,
  2. *Amplicons were only amplified for SSCP analysis; no coding variation was discovered with this method
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BMC Dermatology

ISSN: 1471-5945

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