Exploring the association between Morgellons disease and Lyme disease: identification of Borrelia burgdorferi in Morgellons disease patients

Table 4 Detection of Borrelia DNA by PCR in samples derived from Morgellons patients

#	Specimen	PCR University of New Haven	PCR Australian Biologics	Sequencing
1	Whole callus	pyrG, fla, ospC	16S rRNA	pyrG, fla, ospC
2	Whole callus	pyrG, fla, 16S rRNA	16S rRNA	pyrG
3	Whole callus	fla, pyrG, 16S rRNA, uvrA, ospC	16S rRNA	16S RNA, pyrG, fla, uvrA, ospC
3	Blood culture	fla
4	Callus section	16S rRNA
4	Blood culture	fla
5	Whole callus	pyrG	16S rRNA	pyrG
6	Skin culture	16S rRNA
7	Blood culture	fla
8	Whole callus	pyrG, 16S rRNA, fla		pyrG, fla,
8	Blood culture	fla, pyrG		fla
9	Skin culture	fla		fla
10	Whole callus	16S rRNA, pyrG, fla	16S rRNA	16S rRNA, pyrG
10	Intestinal specimen		16S rRNA
11	Whole callus	fla+/- indeterminate	Inhibited
13	Whole callus	uvrA		uvrA
13	Blood culture	pyrG	16S rRNA
13	Skin culture	pyrG
14	Blood culture	fla	16S rRNA	fla
14	Vaginal culture	fla	16S rRNA	fla
15	Whole callus		16S rRNA
16	Whole callus		rpoC	rpoC
17	Whole callus	pyrG, uvrA	16S rRNA	pyrG, uvrA
17	Blood culture	Real-time 16S rRNA +/- equivocal
18	Whole callus	fla	16S rRNA	fla
21	Skin culture		16S rRNA +/- equivocal
23	Whole callus		16S rRNA	rpoC*
24	Blood culture	fla	16S rRNA
24	Vaginal culture		16S rRNA	rpoC**

*Sequence consistent with B. miyamotoi.
**Sequence consistent with B. garinii.
Amplicon sequences from all patients were consistent with Bb sensu stricto except for sequence from patient 23, which was consistent with B. miyamotoi, and patient 24, which was consistent with B. garinii.