Figure 3

Micrographs of ePUK cultures. 3a: A complete monolayer is shown with ePUK cells in overlying medium. 3b: Adhering keratinocytes cells attached from cells suspended in spent medium when suspension was passed into a new flask. This micrograph was a taken approx. 16 hours after suspension was poured into new flask. 3c: A new monolayer formed after 2–3 days. 3d: A monolayer, after a number of generations (PnSnpnsn) of ePUK culturing demonstrated the very small population of epithelial cells that dominate an optimum monolayer grown using ePUK technology. Bar=50 microns for a,b,c,d.