Figure 1
From: Toll-like receptor 2 activation and comedogenesis: implications for the pathogenesis of acne
TLR Expression in vitro and in vivo . A) TLR2 expression was assessed by RT-PCR in cDNA prepared from HaCaT keratinocytes (first lane), NHEK (second lane) or freshly-isolated human sebaceous glands (third lane, representative of ten different individuals). A no-template cDNA control was included (fourth lane). B) TLR2 antisera were used to evaluate receptor localisation in skin isolated from ten individuals and representative expression patterns are shown, with and without a blocking peptide (pep). Images were captured at 10 × original magnification.