Figure 1

Fibronectin mimics the microenvironment and allows sebocytes to grow in vitro . (a) Scalp sample (9 months old) before microdissection. (b) Isolated sebaceous gland. (c) Immunofluorescence staining on OCT sections of human scalp tissue showed that fibronectin (in red) is expressed in the extracellular matrix surrounding the sebaceous gland. α6-integrin (in green) marked the basal layer of the gland. Boxed area is magnified and shown to (c’). Scale bars, 20 μm (c, c’). (d) Schematic of new method to isolate and cultivate sebocytes. Scalp explants were placed between coverslips coated with fibronectin. Sebaceous gland cells SSG3 growing out of the explant (100x magnification). Abbreviations: SG, Sebaceous Gland, HF, Hair Follicle, FN, Fibronectin.