Figure 4

Reduced mitotic activity and increased senescence in tumours emerging from miRVec-125b transfected Mel-Juso cells shown by (A) ISH with miR-125b (In situ miR-125b), SA-beta-galactosidase staining (X-gal), Ki67 and Cyclin D. The different stainings were not made in paralelle. (B) Quantification of Ki67 and cyclin D expression in murine tumours by visual grading of the proportion of positive cells. In one case the control and experimental tumours were too small for microscopic analysis and therefore the graph shows data for 5 tumours.