Figure 4

Expression of Notch2 , Jagged1 and Manic Fringe genes in untreated and phototherapy treated psoriatic skin. Propidium iodide nuclei counterstaining (A-C, D-F) and in situ hybridisation with radiolabelled riboprobes (A'-C', D'-F'). In psoriatic plaques (A-C'), Notch2, Jagged1 and Manic Fringe genes are down-regulated in the basal layer, and transcripts are mainly located at the tips of the dermal papillae (arrowheads). In psoriatic skin, treated by phototherapy (D-F'), gene expression levels are restored to those of normal control skin (arrowheads). Scale bar, 100 μm; Ma, Manic; d: dermis; bl: basal layer; cl: cornified layer; he: hyperproliferative epidermis.