Figure 5

Notch -related gene expression is down-regulated in psoriatic and in BCC skin biopsies. Using RT-PCR, the amplification rate of a 309 bp fragment from Lunatic Fringe cDNA was compared with that of a 558 bp fragment of GAPDH used as an internal control. One unaffected and three lesional skin cDNA samples were amplified by PCR looking for the following fragments: (Radical Fringe: 300 bp, Manic Fringe: 622 bp, Notch1: 724 bp, Notch2: 473 bp, Delta1: 301 bp, Jagged1: 509 bp). Sample 1: normal human skin; sample 2: skin from an unaffected region of a psoriatic patient; sample 3: psoriatic skin from the same patient; sample 4: psoriatic skin, treated by phototherapy; sample 5 : infiltrating BCC (see Fig. 3C); sample 6: nodular BCC (see Fig. 3B); sample 7:infiltrating BCC (see Fig. 3D,3E,3F); sample 8: unaffected skin surrounding the excised BCC region (see left side of Fig. 3C).