Fig. 3
From: Heat-mediated reduction of apoptosis in UVB-damaged keratinocytes in vitro and in human skin ex vivo
Exposure to UVB plus heat induced a significant decrease in acetylated p53 and an increase in SIRT1 protein levels in NHEK and in skin models. a Immunohistochemical staining of CPD (red), p53-a382 or SIRT1-p (green) and DAPI (blue) in untreated or UVB and/or heat treated ex vivo skin keratinocytes. Inset images are an enlarged view of SIRT1/DNA damaged (CPD positive) keratinocytes, which are also indicated by red arrows. Broken lines denote the epidermal/dermal border. Scale bar (white line) =100 μm. b–e Bar graphs of mean ± SD percent keratinocytes with CPD and positive for (b and c) p53-a382 or (d and e) SIRT1-p per field of view in (b and d) primary NHEK or (c and e) in skin models. Statistically significant differences are indicated with * for p-values <0.05