Fig. 3
Immunohistochemistry findings. a In situ frequency of cytokines and FoxP3 expression (number of positive cells/mm2). Sections of immunohistochemistry staining for (b) anti-IFN-γ (Santa Cruz, Dallas, TX) (c) anti-IL-4 (Santa Cruz), (d) anti-IL-17 (R&D Systems, Minneapolis, MN) and (e) anti-FoxP3 (Ebioscience, San Diego, CA) monoclonal antibodies of a patient’s lesion biopsy. Slides were labeled with streptavidin–biotin complex (Dako, Carpinteria, CA). b and d, original magnification 200×; a and c, original magnification 400×. Numerous stained (brown) cells are found in (b) and (d), a more modest number is found in (e), while rare stained cells (arrow) were detected in (c)